龙牙楤木SE基因克隆与植物表达载体构建

Cloning of Squalene Epoxidase Gene in Aralia elata and Construction of Its Plant Expression Vector

采用RT-PCR的方法,从龙牙楤木中克隆到鲨烯环氧酶(SE)基因,该基因的cDNA全长为1 682 bp,含有1 644 bp的开放阅读框(ORF),编码547个氨基酸,将所得的序列提交GenBank数据库,登录号为GU354314。序列分析结果表明:龙牙楤木SE基因的氨基酸序列与人参、三七、绞股蓝的同源性>90%,并构建了该基因的植物表达载体pAeSE。

The squalene epoxidase （SE） gene from Aralia elata （AeSE） was amplified by RT--PCR. The result showed that the full-length cDNA of AeSE gene （GenBank accession Number： GU354314） is 1 682 bp and contains a 1 644 bp open reading frame （ORF） encoding a polypeptide of 547 amino acids. The SE proteins from Aralia data, Panax ginseng, Panax notoginseng and Gynostemma pentaphyllum showed more than 90 % identities based on amino acid sequences alignment. The plant expression vector pCAMBIA 3301 - AeSE was constructed.