摘要
从不同环境中采集7份土壤样品用于芽孢杆菌的分离纯化,通过多次平板划线分离纯化得到30个菌落形态不同的菌株。以黄瓜枯萎病菌为指示菌对得到的菌株进行抑菌活性筛选,其中的4个菌株具有明显的抑菌作用。采用对峙生长法,测定4株芽孢杆菌对其它植物病原真菌的活性,结果显示4个芽孢杆菌均具有广谱抑菌性。进一步试验表明,4株芽孢杆菌的发酵液也具有明显的抑菌作用,说明对病原真菌有抑制作用的物质水溶性较大,大部分释放到发酵液中,并且发酵液可使黄瓜枯萎等植物病原真菌菌丝膨大、褐变、末端钝圆,促进镰刀菌产生小孢子。通过菌落形态特征、系列生化测定对其进行初步鉴定,结果表明其中一株为枯草芽孢杆菌。
30 Strains of Bacillus spp.were isolated from 7 soil samples using repeated plate streaking,and 4 strains of them had rivalry ability to Fusarium oxysporum f.sp.cucumerinum.They had also antagonism effect and broad spectrum to other plant pathogenic fungi proved by the method of plate test.We detected that the secondary metabolites of Bacillus spp.had fungistasis and could induce deformed growth of hyphae,the metabolites had water-solubility ability.The hyphae of Fusarium oxysporum f.sp.cucumerinum et al.could be induced bulge,browning,tip dulled,produced sporule by zymotic fluid.A strain of Bacillus subtilis was preliminary identified through observation of its morphological features and culture characters and a series of biochemical experiments.
出处
《现代农药》
CAS
2011年第1期44-47,共4页
MODERN AGROCHEMICALS
关键词
芽孢杆菌
拮抗作用
植物病原真菌
发酵液
Bacillus spp.
antagonism
plant pathogenic fungi
zymotic fluid
