叶下珠复方肝丹体外抗HBV作用特点的实验研究

Effect Features of Gandan (A TCM Compound,Majority of Phyllanthus Amarus) on Inhibiting HBV in 2215 Cells

目的探讨叶下珠复方肝丹对ＨＢＶ的体外作用特点。方法通过２．２．１５细胞系模型的改进实验方法，连续每天测定相关数据，动态观察药物对ＨＢＶ的作用情况。结果有如下特征：①有１个药物作用曲线，即从开始用药到见到效果有个过程，需４ｄ～５ｄ，较强的抑制ＨＢＶ作用可维持３ｄ～４ｄ，然后逐渐减弱、消失；②在ＨＢＶ分泌、复制旺盛的时期，药物对ＨＢＶ的抑制作用表现得越强，越能显示出药物的作用；③药物的作用具有呈正比的量效关系。结论体外实验证实肝丹具有抗ＨＢＶ的作用。

To study the pharmacological action of Gandan (a T.C.M. compound medicine which contains Phyllanthus amarus,et al.) on anti HBV,we used the modified experimental method of Transfection Cells (HepG2 2.2.15 cells),which support hepatitis B virus replication, to observe the inhibiting action of Gandan by detecting HBsAg,HBeAg and HBV DNA once a day for 13 days.Results showed:a) There was a similar inhibiting action curve on HBsAg and HBeAg secretion and HBV DNA replication, which showed it needed 3 4 days to introduce the action, the strong action would maintain 3 5 days,then it decreased to disappear gradally. b) The more active HBV replicated,The stronger Gandan inhibiting action was. c) The relationship of drug quantity and its effects was positive correlation.This experiment proved the anti HBV property of Gandan at cellular level and further confirmed its beneficial use in the treatment of hepatitis B.