ω-3转基因克隆绵羊诞生

我们构建了含有bfat-1表达载体pIE-bF1,通过脂质体介导转染获得转基因细胞系,再以核移植技术获得转基因克隆胚胎。胚胎移植后,于2010年10月27日出生两只克隆羊。经检测均为转ω-3基因克隆绵羊。

Expression vector pIE-bF1 with bfat-1 gene was constructed and transfected to ovine fetal fibroblast ceils by liposome protocol. Cloned embryos with these transgenic cells were produced and ansferred to recipient sheep Two lambs were born on October 27, 2010, which are the ω-3 （fat-1） transgenic clones detected by PCR.