摘要
我们构建了含有bfat-1表达载体pIE-bF1,通过脂质体介导转染获得转基因细胞系,再以核移植技术获得转基因克隆胚胎。胚胎移植后,于2010年10月27日出生两只克隆羊。经检测均为转ω-3基因克隆绵羊。
Expression vector pIE-bF1 with bfat-1 gene was constructed and transfected to ovine fetal fibroblast ceils by liposome protocol. Cloned embryos with these transgenic cells were produced and ansferred to recipient sheep Two lambs were born on October 27, 2010, which are the ω-3 (fat-1) transgenic clones detected by PCR.
出处
《内蒙古大学学报(自然科学版)》
CAS
CSCD
北大核心
2011年第1期121-122,共2页
Journal of Inner Mongolia University:Natural Science Edition
基金
教育部科技创新团队基金
内蒙古大学长江学者特聘教授基金
内蒙古自治区重大项目"转基因肉羊新品种培育"项目资助