易激胶囊对腹泻型肠易激综合征模型大鼠血管活性肠肽和肥大细胞表达的影响

Influences of Yiji Capsule on Vasoactive Intestinal Polypeptide and Mast Cells Expression in Diarrhea-predominant Irritable Bowel Syndrome Rats

目的观察腹泻型IBS大鼠模型血浆和肠黏膜组织中血管活性肠肽(VIP)表达及肥大细胞计数的变化。方法采用束缚—制动及乙酸灌肠联合法制备动物模型。SD大鼠分为正常对照组、模型对照组、斯巴敏组和易激胶囊低、中、高剂量组。测定各组大鼠肠道敏感性指标、应用放射免疫分析法(RIA)测定各组大鼠血浆及乙状结肠黏膜内VIP的含量以及各组大鼠盲肠黏膜内MC染色计数。结果各治疗组大鼠肠道敏感性指标均有所改善,易激胶囊能显著降低大鼠血浆及肠道VIP表达及MC细胞计数(P<0.05)。结论易激胶囊可能通过减少IBS模型大鼠结肠黏膜VIP表达及MC的计数,进一步达到治疗IBS的目的。

Objective To observe the vasoactive intestinal polypeptide（VIP） expression in blood plasma and intestinal mucosa as well as the mast cells count in diarrhea-predominant irritable bowel syndrome（IBS） rats.Methods The SD rats were randomized into normal controlgroup,model control group,Spasmomen treated group and low,medium,high dose Yiji capsule treated group after restrain stress and intracolonic instillation of acetic acid.Indexes for intetinal sensitivity,the content of VIP in plasma and sigmoid mucosa of rats and the MC staining in caecum mucosa of rats were measured by RIA.Results The sensitivity indexes in all groups showed improvement.Yiji capsule significantly lowered the blood plasma and intestinal VIP expressions,as well as the MC count（P0.05）.Conclusion The therapeutic effect of Yiji capsule may be realized via reducing expression of VIP in colonic mucosa and MC count.