高效液相色谱法检测枸杞子中吡虫啉残留量

Determination of Imidacloprid Residue in Barbary Wolfberry Fruit by High Performance Liquid Chromatography

目的建立测定枸杞子中吡虫啉残留量的高效液相色谱法。方法采用AT.ChromC18(250mm×4.6mm,5μm)色谱柱,以乙腈-水为流动相进行梯度洗脱,流速为1.0mL/min,检测波长为269nm,柱温为25℃。在样品前处理中采用固相萃取法。结果在0.01-5.0μg/mL间,吡虫啉峰面积与浓度的线性方程为y=30697x+598(r=0.9990),线性关系良好,检测限为0.008μg/mL,且枸杞子中吡虫啉能得到良好分离,重现性好,回收率高。结论本方法简单,快速,结果准确,灵敏度高,且无污染。

Objective To establish a method for the determination of imidacloprid in lycium by high performance liquid chromatography.Methods The analytical column was AT.Chrom C18（250mm × 4.6mm,5μm）,the mobile phase was acetonitrile–water with gradient elution,a flow rate of 1.0mL/min;detection wavelength of 269nm;column temperature 25℃.SPE was used to pre-process the samples.Results Within the range of 0.01-5.0μg/mL,the imidacloprid peak area was observed to be in good linear relationship with its concentration（r=0.9990）,the LOD was 0.008μg/mL,and the imidacloprid in lycium samples can be better separated,with good repeatability and good recovery.Conclusions The method is simple,rapid,accurate and pollution free,therefore is suitable for the determination of imidacloprid in lycium.