海昆肾喜对糖尿病肾病大鼠肾保护及对肾单核细胞趋化蛋白-1及其mRNA表达的影响

Effect of haikunshenxi on the protection for kidney and the monocyte chemoattractant protein-1 and mRNA expression in kidney of diabetic rats

目的:探讨海昆肾喜对糖尿病肾病(diabetic nephropathy,DN)大鼠肾组织单核细胞趋化蛋白-1(MCP-1)及mRNA表达的影响。方法:60只大鼠随机分为正常组10只和造模组50只。造模组采用空腹一次性腹腔注射链脲佐菌素复制糖尿病模型。将造模成功的大鼠按血糖高低随机分为模型组、厄贝沙坦组、海昆小剂量组和海昆大剂量组,给药12周。第4、8、12周末收集大鼠24h尿量并检测尿蛋白量。12周末处死大鼠,取血并分离血清检测肌酐(Scr)、尿素氮(BUN)和甘油三酯(TG);取肾脏采用苏木精-伊红染色、Mallory染色及六胺银染色观察肾组织病理改变,并应用免疫组织化学方法及原位杂交方法检测肾组织MCP-1及mRNA的表达。结果:海昆肾喜能够减少糖尿病大鼠的蛋白尿,增加体重,降低Scr和BUN,改善肾功能,调节TG,改善脂代谢紊乱,并且能够降低糖尿病大鼠肾小管上皮细胞、肾间质细胞内的MCP-1及其mRNA在细胞质内的表达(P<0.01)。结论:海昆肾喜可能抑制MCP-1及mRNA的过表达,进而减缓DN的病程进展。

Objective：To study the expression of monocyte chemoattractant protein-1（MCP-1） and mRNA in the renal tubules of STZ-induced diabetic nephropathy rats.Methods：Sixty SD rats were randomly divided into two groups：normal control（n=10） and diabetic nephropathy group（n=50）.Diabetes was induced by intraperitoneal injection of STZ.And the diabetic rats were randomly divided into model group,irbesartan treatment group,haikunshenxi at low and high doses treatment group.Twenty four-hour urine volume,24-hour urinary protein excretion were examined at weeks 4,8,and 12.All rats were sacrificed at week 12;body weight,kidney weight,and serum excretion,serum creatinine,blood urea nitroge were improved by haikunshenxi preparation.The expression of MCP-1 and mRNA in rat kidney were detected respectively by immunohistochemistry and in situ hybridization.Results：The expression of MCP-1and mRNA in haikunshenxi treatment group was depressed greatly in the renal tubular epithelial cell and renal interstitial cell（P0.01）.Conclusions：Haikunshenxi can inhibit the overexpression of MCP-1 and mRNA in kidney of diabetic rats and delay the progress of diabetic nephropathy.