环境烟草烟雾暴露对小鼠脑区神经递质表达的影响

The influence of experimental tobacco smoke exposure on brain neurotransmitters in mice

目的通过观察小鼠脑部神经递质的变化，初步探讨环境烟草烟雾（ETS）成分对小鼠神经系统的影响。方法在染毒实验台内对小鼠染毒，染毒8周造模后进行小鼠脑区定位，免疫组织学方法和图像分析观察脑区神经递质表达的变化。结果（1）GABA在小鼠大脑皮层、海马部位的表达：ETS暴露组阳性细胞数量明显减少（大脑皮层：0．25±0．10；海马：0．19±0．07）（P〈0．05）。（2）nAChR在大脑皮层的表达：ETS吸入组阳性表达明显增加（0．31±0．10）（P〈0．05）。（3）NMDAR在小鼠大脑皮层、纹状体皮质的表达：ETS吸入组（0．31±0．08，0．35±0．11）、尼古丁（Nic）吸入组阳性表达增加明显（P〈0．05）。结论ETS、Nic可导致大脑皮层、下丘脑等部位神经递质及受体表达的变化：

Objective To explore the mechanism of the influence on mice brain of environmental tobacco smoke. Methods After the mice were placed into the bench for 8 weeks,the region of the mice brain was localized and the expression of neurotransmitters and neurotransmitters receptors were detected by immunobistochernistry. Results （ 1 ） The expression of GABA in the mice cerebral cortex （CC） （0.25 ± 0. 06） and the hippocampus （Hip） （0.19 ± 0.07） were much higher in the ETS-exposed group than that in the control group （P 〈 0.05 ）. （2） The expression of nAChR on CC（0.31 ± 0. 10） was much more in the ETS-exposed group than that in control group （P〈 0.05 ）. （3） The expression of NMDAR（ Glu receptors） on the CC and striate cortex were much higher in the ETS-exposed group（0.32 ±0.10,0.38 ±0.14） ,NIC-inhaling group（0.31 ±0.08,0.31 ±0. 11 ） than that in control group（P 〈 0.05 ）. Conclusions Long-term ETS-exposed and NiC-exposed environment could change the expression of neurotransmitter and its receptors.