摘要
采用超临界CO2流体萃取中药前胡中总香豆素,以石油醚∶乙酸乙酯为洗脱系统,对提取物进行硅胶柱初步分离,利用高效液相色谱确定石油醚∶乙酸乙酯∶甲醇∶水=5∶5∶5∶4作为高速逆流色谱(HSCCC)分离的溶剂体系,在主机转速为850 r/min、流动相流速为2.0 mL/min、254 nm波长检测条件下,根据色谱图手动收集各色谱峰组分,分离所得化合物通过核磁共振(NMR)检测并确定其结构,得到4个单体化合物,分别是前胡香豆素Ⅱ、北美芹素、白花前胡甲素、补骨脂素。
With the extract obtained from radix peucedani by SFE（supercritical fluid extraction）,coumarins were primarily purifed through silica-gel chromatography with solvent system of petroleum ether：ethyl acetate and further purification was processed by HSCCC（high speed counter current chromatography） with solvent system of petroleum ether：ethyl acetate：methanol：water（5∶5∶5∶4） at the flow rate of 2.0 mL/min under rotator speed of 850 r/min.Fractions were collected manually according to HSCCC chromatogram and then charaterized by NMR（nuclear magnetic resonance）,which were peucedanocoumarin II,pteryxin,praeruptorin A and psoralen respectively.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第1期86-89,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家自然科学基金项目(30971951)
湖南农业大学稳定人才基金项目(09WD18)
关键词
白花前胡
高速逆流色谱
前胡香豆素Ⅱ
北美芹素
白花前胡甲素
补骨脂素
Peucedanum praeruptorum
high speed counter current chromatography
peucedanocoumarin Ⅱ
pteryxin
praeruptorin A
psoralen
