3种中草药提取物的多酚含量及抗氧化性

Comparative study on anti-oxidative capacity and polyphenol content of 3 kinds of the extract of Chinese herbal medicine plants

采用Folin-Ciocalteu法对荷叶、枳实和水皂角3种中草药提取物的多酚定量,用分光光度法进行清除自由基能力的评估。结果表明,经过粗提后,多酚含量最高的是枳实提取物,多酚含量为(462.5±19.28)mg/g,其次是荷叶和水皂角提取物,分别含多酚(293.8±78.57)和(270.3±28.53)mg/g。清除DPPH自由基能力最强的是水皂角提取物,其次是荷叶和枳实提取物,IC50值分别为(0.021±0.003)、(0.037±0.007)和(0.061±0.010)mg/mL。抗氧化性测定结果显示,3种中草药的抗氧化性与其酚类物质含量并不呈正相关。

Folin-Ciocalteu and spectrophotometer were adopted to analyse the polyphenol content and DPPH scavenging activityof 3 kinds of extracts from Chinese herbal medicine plants.The results showed that the extract of Citrus aurantum had highest polyphenol contents,which contained（462.5±19.28） mg/g;the second was the extract of lotus leaf and the third was the extract of Cassia normame,containing（293.8±78.57） mg/g and（270.3±28.53） mg/g.The extract of Cassia normame had highest DPPH scavenging activity.The second and the third were the extract of Lotus leaf and Citrus aurantum.The data of IC50 were in the order of（0.021±0.003）,（0.037±0.007） and（0.061±0.010） mg/mL.After comparing the ability of anti-oxidation of 3 herbal medicine plants,it was found that there was no positive relationship between polyphenol contents and anti-oxidative capacity.