大豆栽培种和野生种豆类胰岛素的基因分析

ANALYSIS OF LEGINSULIN GENE IN SOYBEAN CULTIVAR ( GLYCINE MAX ) AND WILD SPECIES ( GLYCINE SOJA ) *

以栽培大豆和野生大豆的基因组ＤＮＡ为材料，利用ＰＣＲ技术扩增并克隆了豆类胰岛素基因，分析了两者的ＤＮＡ序列．结果表明，测定的ＤＮＡ序列分别为８４１ｂｐ和９０３ｂｐ，有４个核苷酸差异；在信号肽编码区域内各存在一个内含子，大小分别为３６８和３７０个核苷酸；在ＯＲＦ范围内，与前人报导的ｃＤＮＡ序列分别有１个和２个位点的核苷酸差异，从而导致相应位点的氨基酸发生变化．Ｓｏｕｔｈｅｒｎ杂交表明。

Leginsulin genes from genomic DNA of soybean cultivar ( Glycine max ) and wild species ( Glycine soja ) were amplified with PCR method, then cloned and sequenced. Results showed that their sizes were 841 bp and 903 bp respectively with 4 nucleotides difference; either of the two genomic clones had one intron, with size of 368 bp and 370 bp respectively in the coding region of its signal peptide ; their DNA sequences had one and two nucleotide difference respectively from cDNA sequence reported previously in its open reading frame which resulted in the amino acid change in the corresponding position. Southern hybridization showed that leginsulin genes of soybean cultivar and wild species existed in the form of single copy and multiple copy respectively in the genome.