摘要
采用DOCA硅胶管皮下埋入法建立大鼠心衰模型,从基因转录水平检测正常与心衰大鼠心肌组织中心肌收缩蛋白分子基因α-MHC、β-MHC、α-cardiacactin、α-skeletalactin表达的变化。结果显示:(1)心衰大鼠心肌收缩力指标dp/dtmax较正常大鼠明显降低(下降27.51%,P<0.01),(2)心衰大鼠与正常大鼠相比,心肌组织中α-MHC基因表达水平显著下降(降低21.43%,P<0.05),β-MHC基因表达水平显著升高(升高62.43%,P<0.01)、α-cardiacactin基因和α-skeletalactin基因表达水平未见明显改变,(3)α-MHCmRNA的含量与心肌收缩力指标dp/dtmax值之间存在正相关关系(r=0.4143,n=43,P<0.05),β-MHCmRNA的含量与dp/dtmax值之间存在负相关关系。(r=-0.3902,n=43,P<0.05)。
The animal model of heart failure was set up by means of deoxycorticosteroneacetate impregnated silicone rubber implants in Wistar rats, and gene expression of myocardial contractile protein, α-MHC、β-MHC、α-cardiac actin、α-skeletal actin in normal and heart failure rats were quantitatively analyzed at gene transcription level. The results showed that: (1)The cardiac contractility index dp/dt max in the heart failure rats is significantly lower than that in the normal (decreased by 27.51% , P<0.01). (2) α-MHC mRNA levels in hearts failure rat heart decreased significantly (21.43%, P<0.05), while β-MHC mRNA levels increased significantly (62.43%, P<0.01), but α-cardiac actin mRNA and α-skeletal actin mRNA levels showed no statistical difference in heart failure and normal rat heart. (3) The α-MHC mRNA level was positively correlated with the dp/dt max value (r=0.4143, n=43, P<0.05), while β-MHC mRNA level was negatively correlated with the dp/dt max value (r=-0.3902, n=43, P<0.05). The above results indicate that changes of MHC gene expression in myocardium is the key molecular basis for the decrease of cardiac contractility during heart failure.
出处
《生物物理学报》
CAS
CSCD
北大核心
1999年第2期400-406,共7页
Acta Biophysica Sinica
基金
国家自然科学基金
关键词
心力衰竭
心肌收缩力
基因
收缩蛋白分子
Heart failure cardiac contractility Contractile protein molecule Gene