不同细胞周期大鼠肝实质细胞癌细胞粘弹特性研究

INVESTIGATION ON THE VISCOELASTICITY OF SYNCHRONOUS HTC CELLS

以胸腺嘧啶核苷和秋水仙碱顺序阻断法及胸腺嘧啶核苷双阻断法分别获得同步化Ｇ１期和Ｓ期细胞，从细胞周期角度出发，采用微管吸吮技术对大鼠肝实质细胞癌细胞的粘弹特性进行了测定并以标准线性固体模型对实验数据进行了拟合，结果表明：该细胞具有高弹性和低粘性的总体特征；Ｇ１期细胞与Ｓ期细胞相比具有高Ｋ１值和低μ值的特点，从而显示Ｇ１期细胞比Ｓ期细胞具有更大的强度和更快的被动变形能力。这些结果不仅反映了同步化细胞存在的细胞骨架状态的周期性差异，也提示Ｇ１期细胞可能比Ｓ期细胞更适于在血流中存活和转移。

Having made use of the micropipette aspiration technique, We here investigated the viscoelastic properties of HTC cells from the view of cell cycle. The synchronous G1 and S phase cells were achieved through thymine-2-desoryriboside and cochicine sequential blockage method and double thymine-2-desoryriboside blockage method respectively. The synchronization results detected with flow cytometer showed that it could meet the requirements of the experiments nicely. A standard linear solid viscoelastic model(Kelvin Model) was adopted to fit the experimental data. The results indicated that high K1, K2 values and low μ value was the general characteristics of the cells; G1 phase cells had higher K1 value and lower μ value than S phase cells had, which endowed G1 phase cells with higher elasticity and faster passive deformability than S phase cells. The results maybe also reflected the difference of cytoskeleton between G1 phase cells and S phase cells, and suggested that G1 phase cells were more suitable for surviving and metastasis in circulation than S phase cells.