金针菇在淀粉工业废水中发酵的生理生化特性

THE PHYSIOLOGICAL AND BIOCHEMICAL CHARACTERISTIC IN FLAMMULINA VELUTIPES SING CULTURE UTILIZING STARCH PROCESSING WASTEWATER AS MEDIUM

以经液化处理的淀粉工业废水作金针菇液体深层发酵的培养基，研究发酵过程中菌球形态、生物量、pH总糖、还原糖、氨态氮、糖化酶、蛋白酶的变化规律，描述了菌体的生长曲线，得到了菌丝体生长的动力学模型。第1～2d为延滞期，第3～8d为快速生长期，第8d后为衰老期。其中第3～6d菌丝体生长最为迅速，酶活力最高，基质消耗最快。发酵过程中pH呈上升趋势。对生理生化各因子之间的相互关系作了讨论。

The liquefied starch processing wastewater was utilized as medium for submerged mycelia culture of Flammulina velutipes. The variation of mycelia pellets morphology, biomass, pH. total sugar, reducing sugar,ammonia nitrogen, amylase activity, protease activity was inveshgated during the process of fermentation. The growth curve for the mycelia of Flammulina velutipes is described and the growth kinehc model has been established. The growth curve showed a lag phase of approximately 2 days followed by a linear phase (the 3rd^8th days) when rapid growth took place, and then followed by a decline phase. During the 3rd-6th days, the speed of mycelia growth reached the top with the highest enzyme achvity and speed of consuming the substrates.The pH value continued to increase during the incubation. The interachon among the physiological and biochemical factors is discussed.