红酵母COS—5产胡萝卜素条件的研究

STUDIES ON CONDITIONS OF CAROTENE PRODUCTION BY Rhodotorula SR COS-5

研究碳源、氮源和添加剂对红酵母（Rhodotorulasp．）COS－5产胡萝卜素的影响，并通过正交试验优化其产胡萝卜素的培养基组成。结果表明，COS－5产胡萝卜素的适宜培养基：蔗糖50g、蛋白胨5g、酵母膏5g、桔子皮15g、盐酸硫胺素0．002g、定容1L，pH6．0.250mL三角瓶装培养基30mL。在上述条件下28℃振荡培养96h，细胞生物量为27．5mg干重／mL发酵液，胡萝卜素含量达492．7μg／g干重细胞。COS－5胡萝卜素在474．4nm、338．6nm和310．2nm处有吸收峰，色素的主要成份为β-胡萝卜素。

Effect of carbon source, nitrogen source and additives on caretene production of stain Rhodotorula sp.COS-5 were inveshgated. The compositions of medium were optimized with orthogonal test The results showed that the optimal medium was as follows (g/L): sucrose 50, peptone 5, yeast except 5, orange skin 15, thiamine 0.002, initial pH6.0. The optimal medium volume in 250 mL flask was 30 mL. 27.5 mg dry cell / mL medium and 492.7 μg carotene / g dry cell were produced by strain COS-5 with above-mentioned conditions after growing at 28℃ for 96h. Carotene produced by stain COS--5 has absorption peaks at 474.4 nm, 338.6 nm and 310.2 nm The major component of pigment of COS--5 is β-carotene.