微生态调节剂抗癫痫敏感性形成作用中神经胶质原纤维酸性蛋白免疫活性变化

CHANGE OF IMMUNOREACTIVITY OF GLIAL FIBRILLARY ACIDIC PROTEIN GFAP IN THE DEVELOPMENT OF MICROECOLOGICAL MODULATOR ON ANTISEIZURE SUSCEPTIBILITY

用红藻氨酸（ＫａｉｎｉｃＡｃｉｄ，ＫＡ）１２ｍｇ／ｋｇ给ＳＤ大鼠颈部皮下注射，诱发动物出现癫痫发作，该癫痫发作于８小时内完全缓解。ＫＡ后１周再次给予ＫＡ（此次为阈下剂量５ｍｇ／ｋｇ），检测上述动物对癫痫刺激的敏感性。结果表明，与对照组比较，于４周前开始并连续灌服微生态调节剂实验组动物癫痫敏感性的形成受到明显抑制（Ｐ＜０００１），同时用免疫细胞化学方法观察大鼠脑内海马部位星形胶质细胞的神经胶质原纤维酸性蛋白（ＧｌｉａｌＦｉｂｒｉｌａｒｙａｃｉｄｉｃｐｒｏｔｅｉｎＧＦＡＰ）免疫反应活性的变化，发现ＫＡ后１～７天，实验组与对照组比较，海马部位神经胶质原纤维酸性蛋白免疫反应活性明显受到抑制。结果表明微生态调节剂的抗癫痫敏感性作用可能与抑制胶质细胞过度增生有关。

Kainic Acid (KA, 12mg/kg) was subcutaneously injected into spragedawlay rats, the seizure was induced and alleviated within 8 hours. One week after KA, the seizure susceptibility was tested with subconvulsive dose of KA(5mg/kg). It showed that the development of the seizure susceptibility in the experimental group which had been previously fed with microecological modulator for four weeks was obviously inhibited (compared to the control group, P<0.001). Meanwhile, immunohistochemistry with monoclonal antibodies against GFAP was used. It revealed that after KA 1-7 day immunoreactivity of GFAP in hipplcamus of the experimental group obviuosly decreased. The results suggested that anti-seizure susceptibility of microecological modulator may be related to its inhibiting the overgrowth of glial cell.\=