一种有效记录人心房肌细胞小电导钙激活钾通道电流的方法(英文)

AN EFFECTIVE METHOD OF RECORDING SMALL CONDUCTANCE Ca^(2+)-ACTIVATED K^+ CHANNELS IN HUMAN ATRIAL MYOCYTE

目的:探讨一种有效的记录人心房肌细胞小电导钙激活钾通道(small conductance Ca2+-activated K+channels,SK)电流的全细胞膜片钳方法。方法:以人心房肌细胞为研究对象,采用传统全细胞膜片钳技术,记录人心房肌细胞的小电导钙激活钾通道的宏观电流。传统全细胞模式形成后,分别记录在浴液中加入apamin(100 nM)前后的电流,两者相减就得到了apamin敏感的SK2的电流。结果:采用传统全细胞膜片钳技术,能有效记录人心房肌细胞上apamin敏感的宏观电流。该电流是内向整流,去极化激活时,无明显失活现象,无尾电流出现,这些都符合SK2电流特性。结论:本实验提供了一种简单、有效的记录人心房肌细胞SK2宏观电流的方法,为今后深入的研究提供了实验基础。

Objective：To establish an effective and stable method of recording small conductance Ca2＋-activated K＋ Channel（SK） currents in human atrial myocytes by conventional whole-cell patch clamp technique.Methods：Conventional whole-cell patch clamp technique was used to record apamin-sensitive currents in isolated right atrial myocytes from human right appendage.After conventional whole-cell configuration was made,apamin（100 nM）-a special blocker of SK2 current was added into extracellular solution.The apamin-sensitive current was obtained by using digital subtraction after apamin from control.Results：SK2 currents in human atrial myocytes were effectively recorded.SK2 current was inward rectification and not obvious effect of inactivation.Conclusion：Conventional whole-cell patch clamp technique can be used to record the SK2 currents.This method provides a basis for future research.