高效液相色谱法测定氨咖黄敏胶囊的含量

Determination in Paracetamol Caffein Artificial Cow-Bezoar and Chlorphenamine Maleate Capsules by HPLC

目的建立同时测定氨咖黄敏胶囊中对乙酰氨基酚和咖啡因含量的高效液相色谱法。方法色谱柱为Aglient C18柱(250 mm×4.6 mm,5μm),流动相为1%醋酸溶液(用乙二胺调pH至3.7)-甲醇(60:40),流速为1.0 mL/min,检测波长272 nm,柱温30℃。结果对乙酰氨基酚、咖啡因进样量分别在0.122 2～3.055 5μg和0.007 482～0.187 1μg范围内与峰面积呈良好线性关系,r分别为1.000 0和1.000 0(n=6),平均加样回收率分别为99.54%(RSD=0.66%)和100.43%(RSD=0.92%)。结论该方法快速简便、准确可靠,可用于氨咖黄敏胶囊的质量控制。

Objective To establish a HPLC method for simultaneously determining paracetamol and caffiene in Paracetamol Caffein Artificial Cow-Bezoar and Chlorphenamine Maleate Capsules.Methods The analytical column was Aglient C18（250 mm×4.6 mm,0.5 μm） with the column temperature of 30 ℃.The mobile phase was 1% acetic acid（adjusting to pH 3.7 with ethylenediamine）-methanol（60 ∶40） with flow rate of 1.0 mL/min.The detection wavelength was set at 272 nm.Results The linear ranges of paracetanol and caffeine were 0.122 2-3.055 5 μg and 0.007 482-0.187 1 μg with the relative coefficient r=1.000 0,r=1.000 0（n=6）,respectively.The average recovery rates of paracetamol and caffeine were 99.54%（RSD=0.66%）and 100.43%（RSD=0.92%）,respectively.Conclusion The method is simple,rapid,accurate and reliable,which can be used for the quality control of Paracetamol Caffein Artificial Cow-Bezoar and Chlorphenamine Maleate Capsules.