黄芪注射液对梗死灶周脑组织HIF-1α和VEGF的影响

The Effect of Astragalus Injection on the expression of HIF-1α and VEGF in the Cerebral Tissue after Focal Cerebral Ischemia

目的:观察黄芪注射液对缺血性脑损伤大鼠脑组织低氧诱导因子(HIF-1α)和血管内皮细胞生长因子(VEGF)影响,探索黄芪注射液对缺血脑组织血管生成的调节作用。方法:30只SD大鼠随机分为假手术组,模型组和黄芪注射液组。采用大脑中动脉局灶性栓塞模型,RT-PCR法检测缺血脑组织中HIF-1α和VEGF改变。结果:与模型组相比,黄芪注射液可显著升高缺血脑组织HIF-1α和VEGF表达(P<0.01)。结论:黄芪注射液可以通过上调缺血脑组织HIF-1α和VEGF的表达起到脑保护作用。

Objective To observe the effects of astragalus injection on the expression of HIF-1α and VEGF in the cerebral tissue after focal cerebral ischemia reperfusion in rats.Methods Thirty male SD rats with average body weight of 260-300g were randomly divided into three groups： sham-operation group,ischemic model group and astragalus injection group.The model of the middle cerebral artery occlusion in rats was performed with the suture occlusion for two hours.The expression of HIF-1α and VEGF were detected by using reverse transcription-polymerase chain reaction（RT-PCR）.Results Compared with sham-operation group,the expression of HIF-1α and VEGF increased in the ischemic model group,and compared with ischemic model group,astragalus injection could significantly raise the expression of HIF-1α and VEGF（P〈0.01 and P〈0.05,respectively）.Conclusions These results suggested that the astragalus injection ccould significantly protect cranial nerve in ischemic penumbra around ischemia reperfusion cerebral tissue in rats.It may through up-regulating the expression of HIF-1α and VEGF.