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葡萄糖3-脱氢酶产生菌的筛选和产酶培养

Screening of glucoside 3-dehydrogenase producing strains and its fermentable condition
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摘要 从土壤中筛选分离得到1株葡萄糖3-脱氢酶产生菌,经生理生化初步鉴定为产酸克雷伯菌(Klebsiella oxyto-ca)。将菌株D8进行产酶优化培养,经过单因素法确定优化培养条件:乳糖为C源,酵母膏为N源,250 mL摇瓶装液量为50 mL,培养基的初始pH为7,33℃培养时G3DH酶比活力最高,达到1.45 U。 A glucoside 3-dehydrogenase producing was isolated from soil.The strain was identified as Klebsiella oxytoca.The culture condition was optimized by single factor experimental design.The activity of glucoside 3-dehydrogenase produced by this strain was 1.45 U under liquid fermentation conditions： carbon source,lactose,nitrogen source,yeast extract,50 mL culture medium in 250 mL shaking flask at 33 ℃,pH 7.0.
作者 张建芬 沈明杰 曾为锋 季佳 连振静
机构地区 浙江树人大学生物与环境工程学院
出处 《生物加工过程》 CAS CSCD 2011年第1期41-45,共5页 Chinese Journal of Bioprocess Engineering
基金 浙江省大学生科技成果推广项目(2009) 浙江树人大学人才引进科研项目(2008R001)
关键词 葡萄糖3-脱氢酶 产酶培养 KLEBSIELLA oxytoca glucoside 3-dehydrogenase fermentable condition Klebsiella oxytoca
分类号 Q814 [生物学—生物工程]
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参考文献11

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