牛皮消及炮制品HPLC指纹特征及含量测定研究

Determination and HPLC fingerprint characteristics of crude and processed 'Niupixiao'

目的:比较牛皮消饮片生品及米炒炮制品的HPLC指纹特征,为二者提供鉴定依据;对炮制前后所含成分A(对甲基苯酚)和成分B[17-O-acetyl-kidjoranin-3-O-β-D-digitoxopyrayl-(1→4)-β-D-digitoxopyrayl-(1→4)-α-L-cymaropyranosyl-(1→4)-β-D-diginopyranosy(1→4)-α-L-cymaropyranoside]进行含量测定研究,探讨炮制意义。方法:反相高效液相色谱法及中药指纹图谱研究方法。色谱条件:COSMOSIL 5C18-MS-Ⅱ色谱柱(4.6mm×250mm,5μm),乙腈-水溶液梯度洗脱,流速0.3-1.0mL/min,柱温25℃,检测波长210nm。结果:炮制前后指纹图谱差异明显;炮制后具有刺激性的成分A的含量显著降低。结论:建立的指纹图谱及含量测定方法为炮制前后饮片鉴别与质量控制提供了重要依据,并初步说明炮制对降低毒副作用有一定意义。

Objective： To compare the HPLC fingerprint characteristics between the crude and processed ‘Niupixiao’in order to provide a scientific basis for identification,and to make a preliminary study about significance of the processing through determination of component A（p-cresol） and B [17-O-acetyl-kidjoranin-3-O-β-D-digitoxopyrayl-（1→4）-β-D-digitoxopyrayl-（1→4）-α-L-cymaropyranosyl-（1→4）-β-D-diginopyranosyl（1→4）-α-L-cymaropyranoside] in the crude and processed ‘Niupixiao’.Methods： Methods of HPLC and TCM fingerprint were both applied in this study.The separation was performed on COSMOSIL 5C18-MS-Ⅱ column（4.6mm×250mm,5μm）,using water-acetonitrile gradient elution,with a flow rate of 0.3-1.0mL/min.The detection wavelength was 210nm.Results： The fingerprint characteristics between them showed apparent differences.Component A with side effects has significantly reduced by processing.Conclusion： The HPLC fingerprints established can be used for distinguishing crude and processing ‘Niupixiao’,and illustrated that the processing can reduce toxicity.