海带对实验性高脂血症大鼠血脂水平的调节作用机制

Regulating effects and mechanism of Laminaria japonica on serum lipid of experimental hyperlipemia rats

目的:探讨海带对高脂血症大鼠血脂的调节作用机制。方法:健康雌性Wistar大鼠40只,应用高脂饲料喂养方法建立高脂血症动物模型,海带粉饲料喂养干预治疗2周。生化法检测大鼠血清甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平及脂蛋白酯酶(LPL)和肝脂酶(HL)活性。酶联免疫吸附法测定羟甲基戊二酰辅酶A(HMG-CoA)水平和羟甲基戊二酰辅酶A还原酶(HMG-CR)活性。结果:经海带干预治疗后,动物血清TG、TC和LDL水平较模型对照组显著降低(P<0.05),而HDL水平显著升高(P<0.05)。海带治疗组动物血清和肝组织中LPL和HL水平均显著高于模型对照组(P<0.05),而显著低于阳性对照组(P<0.05)。海带治疗组动物肝组织HMG-CoA的含量显著高于模型对照组、而HMG-CR的活性显著低于模型对照组(P<0.05),且HMG-CR活性显著高于阳性对照组(P<0.05)。结论:海带可能通过增强LPL、HL和HMG-CR的活性,影响TG、TC、LDL和HDL等组分的代谢,发挥调节血脂水平的作用。

Objective： To investigate the regulating effects and mechanism of Laminaria japonica on serum lipid of hyperlipemia in rats.Methods： Forty healthy female Wistar rats were used to establish hyperlipemia models by feeding fat-rich forage,and the Laminaria japonica was applied as raw materials for potential marine drugs.The levels of serum lipid including the triglyceride（TG）,total cholesterol（TC）,low-density lipoprotein（LDL）,high-density lipoprotein（HDL） and the activity of lipoproteinesterase（LPL） and hepatic lipase（HL） were detected by biochemical assay.Enzyme-linked immunosorbent assay（ELISA） was applied to determine the level of 3-hydroxy-3-methylglutaryl coenzyme A（HMG-CoA） and activity of HMG-CoA reducase（HMG-CR）.Results： After treated with Laminaria japonica,the levels of serum TG,TC and LDL decreased and the serum HDL increased significantly than those in model group rats（P0.05）,but no difference between positive group and Laminaria japonica group.The levels of LPL and HL in serum and hepatic tissue in Laminaria japonica group rats were significantly higher than those in model group rats（P0.05） and lower than those in positive group（P0.05）.The HMG-CoA level of hepatic tissue in Laminaria japonica group rats was significantly higher and the HMG-CR activity lower than those in model group（P0.05）,while the activity of HMG-CR in Laminaria japonica group was higher than that in positive group（P0.05）.Conclusion： Laminaria japonica might play regulating effects on serum lipid by enhancing the activities of LPL and HL and inhibiting the activity of HMG-CR to interfere the metabolism of TG,TC,LDL and HDL.