针灸对脑梗死大鼠患侧海马CA1区Bax的影响

The Effect of Acupuncture on Bax of Hippocampal Neurons in Ischemia Side and the Relationship Between the Effect and Learning and Memory in Cerebral Infarction Rats

目的:观察针灸治疗对脑梗死大鼠患侧海马CA1区Bax蛋白表达量及学习记忆能力的影响。方法:将成功制成右侧大脑中动脉缺血梗死模型的48只大鼠随机分为脑梗死自由活动组(对照组)和脑梗死针灸组(针灸组)2组,每组各24只。用免疫组化方法检测其Bax蛋白表达量及凋亡指数,用Y-迷宫测试其学习记忆能力。结果:第1周、第2周、第3周各时段针灸组大鼠与对照组比较,学会Y-迷宫所花的次数较少,Bax蛋白阳性神经元数较少,患侧海马CA1区凋亡指数较低,均有显著性差异(P<0.05)。结论:①针灸可减少海马Bax蛋白的表达,减少细胞凋亡,海马最终得以保存更多神经元而发挥其改善学习记忆功能的作用。②针灸能明显改善脑缺血大鼠的学习记忆能力。

Objective：To observe the effect of acupuncture on Bax of hippocampal neurons in ischemia side and Learning and Memory in cerebral infarction rats.Methods：Of 80 male rats,48 rats were made into cerebral infarction models from by occlusion of the middle cerebral artery with clew.These were then divided randomly into 2 groups：control group（24 rats） and acupuncture group（24 rats）.The expression of Bax of hippocampal neurons in ischemia side were detected by immuno-histochemistry,and the ability of Learning and Memory were evaluated by Y-maze.The data were analyzed by one-way analysis of variance,T-test and q test.Results：In 1,2 and 3 weeks group,the times of Y-maze,the positive expression of Bax and apoptosis index in hippocampus CA1 region in control groups exceeded that of acupuncture groups（P0.05）.Conclusion：The ability of learning and memory in cerebral infarction rats was improved by acupuncture,which may be accomplished by decreasing Bax expression in hippocampus CA1 region,then protecting hippocampus cells from apoptosis.