紫彩血蛤鳃的组织化学和扫描电镜研究

HISTOCHEMICAL AND ELECTRON SCANNING MICROSCOPIC STUDIES ON THE GILLS OF NUTTALLIA OLIVACEA

紫彩血蛤（Ｎｕｔａｌｉａｏｌｉｖａｃｅａ）的鳃上皮细胞核多为椭圆形，核内染色质呈丝状；结缔组织细胞的核多为圆形，部分核内有块状Ｆｅｕｌｇｅｎ阳性颗粒。ＲＮＡ在鳃丝上皮细胞中最丰富，有的形成直径０５２μｍ的颗粒。结缔组织和鳃丝上皮细胞内均有直径０６３μｍ左右的ＰＡＳ阳性颗粒。吞噬细胞呈极强的ＡＮＡＥ阳性，鳃丝上皮细胞呈较弱的阳性。ＡＣＰ和ＡＫＰ在吞噬细胞和外缘上皮细胞中活性较强，其它部位较弱。ＭＰＯＤ只在鳃丝基部呈强阳性。扫描电镜观察，鳃表面密生纤毛，主鳃丝处形成沟，１８～２１条鳃丝形成一条状隆起。鳃纤毛间夹着许多杯状细胞，有的杯状细胞外可见圆形分泌泡。

Most epithelial nuclei of Nuttallia olivacea are elliptic in shape and the chromatin is filiform.Most nuclei of connective tissue are round and have Feulgen positive granules.RNA is rich in the epithelial cells of gill filaments.RNA granules 0 52μm in diameter are observed.There are PAS positive granules about 0 63μm in diameter in the cells of connective tissue and the epithelial cells of filaments.Phagocytes present very strong ANAE positivity while the epithelial cells present weak positivity.The activeness of ACP and AKP are strong in phagocytes and outer epithelial cells but weak in other cells.MPOD is strong positive only at the bases of filaments.Scanning electron microscopic observations show that cilia grows densely on the surface of gill.Gullis are located in the main filaments.18～21 filaments form a ridge.There are many cupped cells among cilia of gill and some round secretory vacuoles outside of some cupped cells.