雾化吸入NO供体对新生猪急性呼吸窘迫综合征炎症反应的干预作用

Modulatory effects of nebulized nitric oxide donor on inflammatory response in newborn piglets with acute respiratory distress syndrome

目的探讨雾化吸入NO供体对急性呼吸窘迫综合征炎症反应的干预作用机制。方法健康出生后3—5d的新生猪45只随机分成5组，A组为对照组：气管插管，接呼吸机；B组为NS组：模型建立后，经呼吸机管道雾化吸人生理盐水30rain；C组为模型建立后，经呼吸机管道雾化吸入Neb—SNP（1rag／m1，0．9％NaCl配制）30min；D组为模型建立后，经呼吸机管道雾化吸入Neb—SNP（5mg／m1）30min；E组为模型建立后，经呼吸机管道雾化吸入Neb—SNP（10mg／ml）30min。各组分30、60、120min3个时间点各3只测定肺组织核因子（NF）-κB的表达及血清细胞因子TNF—α、IL-12、IL-10的浓度。结果治疗组肺组织NF—KB表达显著降低（P〈0．05），血清TNF-α、IL-12浓度显著降低（P〈0．05），IL—10浓度明显增高（P〈0．05）；Neb—SNP雾化浓度增加，NF-KB表达下降，炎症因子TNF-α、IL-12浓度下降，IL-10浓度升高[C组与D组差异有显著性（P〈0．05），D组与E组差异无显著性（P〉0．05）]。结论Neb—SNP通过下调NF—κB的活性，抑制炎症因子的表达从而减轻急性呼吸窘迫综合征的肺损伤。

Objective To investigate modulatory effects of Neb-SNP on inflammatory response and to explore the protection mechanisms of Neb-SNP in newborn piglets with ARDS. Methods Forty-five neonatal swines were randomly divided into five groups：group A （controlled group, n = 9 ）, group B （physiological saline group,n =9）,group C （Neb-SNP 1 mg/ml,0. 9% NaCl,n =9）,group D （Neb-SNP 5 mg/ml, 0. 9% NaCl, n = 9 ）and group E （Neb-SNP 10 mg/ml,0. 9% NaCl, n = 9 ）. The pathological changes and activity of NF-κB in the lung tissue,TNF-α,IL-10 and IL-12 concentrations in serum at 30 minutes,60 minutes and 120 minutes after aerosol inhalation were observed. Results Activity of NF-κB and serum concentrations of TNF-α and IL-12 in the Neb-SNP treated group were lower than group B（P 〈0. 05） ,and serum IL- 10 concentration was obviously higher in the Neb-SNP group（ P 〈 0. 05 ）. With an increase of Neb-SNP concentration, activity of NF-KB and serum concentrations of TNF-α and IL-12 were obviously increased, while serum concentrations of IL-10 was increased in group D and group E than that of group C （ P 〈 0. 05 ）. Conclusion Inhalation of Neb-SNP reduced lung injury induced ARDS through lowering NF-κB activity and inhibiting expression of harmful inflammatory cytokines.