人参皂甙Rb1对大鼠脑缺血再灌注后巢蛋白表达的影响

Expression of nestin in rat following cerebral ischemia-reperfusion and effects of ginsenoside Rb1 on it

目的:观察大鼠脑缺血再灌注后脑组织内巢蛋白的表达以及人参皂甙Rb1(Ginsenoside Rb1,GRb1)对神经的保护机制。方法:阻塞大鼠大脑中动脉2h制备脑缺血再灌注模型,大鼠随机分为缺血再灌注组(I/R组)和GRbl给药组(GRbl组)。两组按不同的再灌注时间(3h、12h、1d、2d、3d、5d、10d)分为7个亚组,应用免疫组织化学技术检测脑内巢蛋白的表达。结果:与I/R组相比,GRbl能减轻脑组织的病理改变,上调各时间点巢蛋白阳性细胞数。结论:GRbl对脑缺血再灌注损伤有神经保护作用,其机制与上调缺血再灌注大鼠脑内巢蛋白表达水平有关。

Objective：To observe the effects of ginsenoside Rb1（GRb1） on the expressions of nestin in rats after cerebral ischemia-reperfusion so as to investigate the neuroprotective mechanism of GRb1.Methods：The model of cerebral ischemia-reperfusion was established by occluding rat middle cerebral artery for 2 h.The rats were randomly divided into two groups： ischemia-reperfusion group（I/R group）and GRbl treat group（GRbl group）.The two groups were further subdivided into 7 subgroups according to 3h,12 h,1 d,2 d,3 d,5 d and 10 d,n=4 per time point）.Expression of nestin was detected with an immunohistochemical approach.Results：Compared with I/R group,GRbl reduced the pathological changes in brain tissues,and up-regulated the number of nestin positive cells.Conclusion：GRbl has the neuroprotective effect on cerebral ischemia-reperfusion injury,its mechanism is related to up-regulate the expression of nestin.