山豆根多次给药肝毒性机制探讨

The Research on Mechanism of Liver Toxicity Caused by Radix et Rhizoma Sophorae Tonkinensise

目的研究山豆根不同组分多次给药致小鼠肝脏毒性与氧化损伤的相关性。方法连续7天给小鼠灌胃不同剂量的山豆根水提、醇提组分,分别于给药后第1、3、7天检测小鼠血清中超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)、谷胱甘肽过氧化物酶(GSH-Px)的活性和丙二醛(MDA)、一氧化氮(NO)、谷胱甘肽(GSH)的含量。结果山豆根水提、醇提组分可导致血中SOD、GSH-Px活性下降,GSH含量下降,NOS活性上升,MDA、NO含量增加。上述变化随剂量增加、给药时间的延长而逐渐加重。结论连续多次给予山豆根水提组分、醇提组分导致小鼠肝毒性损伤的途径与过氧化损伤机制有关。

Objective To investigate the mechanisms of liver toxicity induced by different components from Radix et Rhizoma Sophorae Tonkinensise in mice.Methods Mice are divided into different groups such as normal,water extract and alcohol extract group to observe the SOD、NOS、GSH-Px、MDA、NO and GSH separately on the d1,d3,d7 after seven days administration.Results The SOD、GSH-Px and GSH were decreased;while the NOS,MDA,NO were increased after given water extract and alcohol extract from Radix et Rhizoma Sophorae Tonkinensise to mice.These changes were enhanced with the increasing of dose and time.Conclusion Multiple intragastric water extract and alcohol extract of Radix et Rhizoma Sophorae Tonkinensise with certain dosage can induce acute hepatotoxic injury in mice,and it is related to the peroxidation.