冠心合剂对心梗后大鼠VEGF表达的影响

Effects of Guanxin Decoction on the Expression of VEGF in Rat of Acute Myocardial Infarction

目的:观察冠心合剂对急性心肌梗死(AMI)大鼠血管内皮生长因子(Vascular endothelial growth fac-tor,VEGF)表达的影响。方法:结扎冠状动脉左前降支法建立大鼠急性心肌梗死模型,观察冠心合剂对心梗后大鼠血清VEGF浓度、心肌VEGF蛋白表达及梗死面积的影响。结果:冠心合剂治疗组及阳性对照组血清VEGF浓度及心肌VEGF蛋白的表达增加,高于模型组和假手术组(P<0.05);随冠心合剂剂量的增加,梗死面积逐渐减小;冠心合剂大剂量组疗效与西药对照组无明显差异(P>0.05)。结论:冠心合剂可能通过促进心肌梗死大鼠心肌VEGF蛋白表达、分泌,从而促进梗死心肌血管新生。

Objective ：To investigate the effect of Guanxin Decoction on myocardial angiogenesis and the expression of VEGF in rats with acute myocardial infarction. Methods： Left anterior descending coronary arteries （LAD） have been ligated in SD rats after anesthetized by 2.5 % pentobarbital sodium. The level of VEGF in blood serum was measured 2 weeks after the experiment by ELISA respectively. The heart specimen were fixed, sectioned and colorized by means of HE staining and staining to observe VEGF through microscope. Results： Guanxin Decoction - treatment group and positive control group were higher than the model group and the sham - operation group in the expression of VEGF protein （ P 〈 0.05 ）. The level of VEGF in blood serum increased correspondingly with the increase of the dose of Guanxin Decoction, while the myocardial infarction scope reducing. The curative effect of the high - dosage Guanxin Decoction is similar with bFGF and heparin（ P 〉 0.05 ）. Conclusion： The mechanism of AMI rat angiagenesis which is prorooted by Guanxin Decoction may be associated with the increase of expression and excretion of VEGF in cardiac myocytes.