开郁清热方干预自发2型糖尿病大鼠腹腔脂肪组织SREBP-1c、FAS表达的实验研究

The Experiment Study of Kaiyuqingre's Prescription on the Expression of Sterol Regulatory Element Binding Protein-1c and Fatty Acid Synthase in Peritoneal Adipose Tissue of Spontaneous Type 2 Diabetes Mellitus Rats(OLETF rats)

目的:研究开郁清热方对自发2型糖尿病大鼠(OLETF大鼠)腹腔脂肪组织SREBP-1c、FAS蛋白及mRNA表达的影响。方法:将成模OLETF大鼠随机分为模型组、二甲双胍组、开郁清热方组,以LETO大鼠为空白对照组。采用免第疫组化、RT-PCR法检测腹腔脂肪组织SREBP-1c、FAS蛋白及mRNA的表达。结果:开郁清热方组的脂肪组织SREBP-1c、FAS蛋白及mRNA表达水平较模型组明显减低(P<0.01,P<0.05)。结论:开郁清热方具有降低自发2型糖尿病大鼠脂肪组织SREBP-1c、FAS蛋白及mRNA表达的作用。

Objective： To observe the effect of Kaiyuqingre＇s Prescription on the protein and mRNA expression of sterol regulatory dement binding protein - lc and fatty acid synthase in peritoneal adipose tissue of spontaneous Type 2 Diabetes Mellitus rats（OLETF rats）. Methods ：A control study was carried out between the OLETF rats and LETO rats,and all OLETF rats were divided into three groups randoraly：Modd group,Metfomin group and Kaiyuqingre＇s Prescription group. method and real-time floureseent quantitative polymerase chain reaction（ PCR）technology were used to detect the expression of sterol regulatory dement binding protein - lc and fatty acid synthase in adipose tissue from the protein and gene levels in each group. Results： The sterol regulatory dement binding protein - 1c and fatty acid synthase protein and mRNA expression in rats adipose tissue：Contrast to Modal group,the Kaiyuqingre＇s Prescription group is significantly lower. Conclusion ：Kaiyuqingre＇s Prescription has a role of reducing the expression of protein and mRNA of sterol regulatory dement binding protein - 1c and fatty acid synthase in adipose tissue of spontaneous Type 2 Diabetes Mellitus rats.