山茱萸环烯醚萜苷对脑缺血沙土鼠学习记忆能力和脑内神经营养因子的影响

Cornel Iridoid Glycoside Improves Learning-memory Ability and Increases Expression of Neurotrophic Factors in Global Brain Ischemic Gerbils

目的：观察山茱萸环烯醚萜苷（CIG）对脑缺血动物学习记忆能力以及海马区BDNF蛋白表达的影响。方法：沙土鼠双颈总动脉夹闭阻断血流5min造成全脑缺血模型。4～5周后，通过避暗试验和Morris水迷宫试验观察动物学习记忆能力；应用尼氏染色法检测海马区神经元的损伤程度；免疫组织化学和免疫印迹方法检查脑海马BDNF的表达情况。结果：灌胃给予CIG可以明显改善全脑缺血沙土鼠的被动回避记忆和空间学习谒忆能力，显著提高海马区BDNF的表达，保护CA1区神经元细胞免受缺血性损害。结论：CIG具有神经保护作用，其机制可能与促进海马区神经营养因子的表达有关。

Objective： To investigate the effects of Cornel iridoid glycoside （CIG）on the learning and memory ability and neurotrophic factors in the brain of global ischemic gerbils. Methods ：The global brain ischemic model was made by occluding common carotid arteries for 5 min in gerbils. After 4 to 5 weeks, passive avoidance memory and spatial learning - memory abilities were performed by step through and Morris water maze tests, respectively. Nissl staining was used to observe the survival neurons in hippocampal CA1 area. The expression of brain - derived neurotrophic factors （BDNF） was detected with immunohistochemical staining and western blotting. Results ： Intragastrical administration of CIG significantly improved the passive avoidance memory and spatial learning - memory abilities, increased the expression of BDNF in hippocampal CA1 area and protected CA1 neurons against ischemia damage in gerbils. Conclusions ：GIG can improve the cognitive function of gerbils suffered from global brain ischemia. The mechanism may involve increasing the expression of BDNF, thereby protecting CA1 neurons against ischemie insults.