膝骨关节炎大鼠丘脑及下丘脑β-EP受体含量变化及针刀松解法的影响研究

Research on the Change of β-EP Receptor Content in Thalamus and Hypothalamus of Rats with Knee Osteoarthritis and the Effect of Acupotomy Lysis

目的:通过观察针刀松解法干预后丘脑及下丘脑β-EP受体含量的变化并与电针干预相比较,探讨针刀松解在脑内的镇痛机制。方法:将60只健康SD大鼠随机分为空白组、模型组、电针组和针刀组。将4%木瓜蛋白酶溶液与0.3mol/L半胱氨酸溶液按1∶1混合静置0.5h后分别于造模第1、4、7天注射于模型组、针刀组和电针组大鼠左膝关节腔,每次20μL。4周后针刀组和电针组给予相应治疗。治疗3周后大鼠断头处死,取出脑组织,分别测定丘脑、下丘脑的β-EP受体含量及其亲和常数。结果:在丘脑及下丘脑模型组、针刀组、电针组的β-EP R1含量有升高的趋势,在丘脑三组与空白组相比差异显著(P<0.05)。无论在丘脑或是下丘脑,β-EP R2含量的变化都不十分明显,组间比较均无显著性差异(P>0.05)。亲和常数方面膝骨关节炎形成过程中β-EP受体亲和常数表现为升高的趋势。在丘脑,模型组β-EP R1及β-EP R2亲和常数的变化与空白组相比有显著性差异(P<0.05);在下丘脑水平β-EPR1的变化趋势较β-EP R2明显,模型组β-EP R1的亲和常数显著高于空白组(P<0.05)。针刀松解或电针干预后模型大鼠的β-EP受体亲和常数无明显变化,与模型组相比无显著性差异(P>0.05);而与空白组相比存在显著差异(P<0.01,P<0.05)。结论:针刀松解法对膝骨关节炎大鼠丘脑及下丘脑β-EP受体含量无明显的影响。在膝骨关节炎的形成过程中痛刺激使得β-EP受体的亲和力升高,这有利于镇痛作用的发挥。针刀松解法一方面通过局部作用降低了痛刺激信号向中枢的传入,另一方面维持了β-EP受体亲和力升高的趋势,这可能是针刀松解法镇痛的机制之一。

Abstract： Objective： To study the analgesia mechanism of aeupotomy lysis （AL） inside the brain by comparing the change of β - EP receptor content in thalamus and hypothalamus of Knee Osteoatthritis（KOA） rats that have undergone AL with that of rats treated with electro -acupuncture （EA）. Methods： Divide 60 healthy SD rats randomly into normal control group, model group, electro -acupuncture （EA） group , and acupotomy lysis （AL） group. Mix 4% papain solution with 0.3mol/L cysteine solution in the ratio of 1 ： 1. Pause for 0.5h, iniect the mixture, 20μL each time, into the left knee joint cavities of rats in model, AL, and EA groups on days 1,4, 7. After 4 weeks treat AL and EA groups rats. After 3 weeks of treatment, behead the rats and take samples of brain, measure respectively the β - EP receptor content in thalamus and hypothalamus, and its affinity constant. Compare the value of AL group respectively with the values of normal control group, model group, and EA group. Results： The β -EPR1 content in thalamus and hypothalamus of rats in model group, AL group, and EA group show a tendency to rise. There is clear difference between the β - EPR1 content in thalamus of rats in normal control group and the other three groups and （ P 〈 0.05 ）. No noticeable change of β - EPR2 content in thalamus and hypothalamus is found, nor significant difference between any two groups. With respect to affinity constant, during the formation of KOA the affinity constant of β - EP receptor shows a tendency to rise. The change of affinity constant of β - EPR1 and β - EPR2 in thalamus of rats in model group is significantly different （ P 〈 0.05） than that of rats in normal control group. In hypothalamus, β - EPR1 shows a stronger tendency to rise than β - EPR2. The affinity constant of model group β - EPR1 is much higher than that of normal control group （ P 〈 0.05 ）. No noticeable change of β - EP affinity constant of rats is found after AL or EA interference, nor significant difference compared with modal group （ P 〉 0. 05）. While significant difference exists compared with normal control group （ P 〈 0.01, P 〈 0.05）. Conclusion： AL has no conspicuous effect on β - EP receptor content in thalamus and hypothalamus of rats with KOA. During the formation of KOA the affinity of receptor has a tendency to rise, that helps ease pain. AL can-not strengthen the tendency to rise, its functions to regulate in a positive manner the β - EP content in different parts of central nervous system of rats with KOA, that also helps ease pain.