补肾活血合剂对糖尿病阳痿大鼠阴茎平滑肌组织中Bcl-2 Bax和Caspase-3表达的影响

The effects of the Reinforcing Kidney and Activating Blood Recipe Affecting the Value of bc1-2,bax and caspase-3 in the Tissue of Corpus Cavernosum Smooth Muscle of DMED-Rats

目的:观察补肾活血合剂对糖尿病阳痿(DMED)大鼠阴茎平滑肌组织中Bcl-2、Bax、Caspase-3表达的影响。方法:将实验小鼠用2%链脲佐菌素液(STZ)按60mg/kg,腹腔注射建立糖尿病模型,然后在每只糖尿病模型大鼠颈项处注射阿朴吗啡(AP0sigma公司)80μg/kg,录相记录阴茎勃起次数。筛选DMED模型,将造成DMED模型随机分为正常组、模型组、中药高、低剂量组、达美康组、达美康+安雄组。除正常组外,其余各组连续给药12周,然后处死测定阴茎平滑肌组织中Bcl-2,Bax,Caspase-3的表达情况。结果:补肾活血合剂对Bcl-2,Bax,Caspase-3有改善作用。结论:补肾活血合剂对糖尿病阳痿大鼠阴茎平滑肌组织中Bcl-2,Bax,Caspase-3有调控效果。

Objective ： Observing the effects of reinforcing kidney and activating blood recipe affecting the value of bcl - 2, bax and caspase - 3 in the tissue of corpus cavernosum Smooth muscle of DMED - rats. Methods ： An animal model of diabetes was induced by a single intravenous dose of streptozotocin （STZ, 60mg/kg body weight） in Wistar rats. Re- cording the numbers of penile erection of each rat by camera after subcutaneous inject of apomorphine （80mg/kg）. Screening the model of STZ - rats with erectile dysfunction and grouping into 6 groups ： control group, model group, Chi- nese Medicine group, low - dosage group, Diamicron group, Diamicron combined Testosterone Undecanoate group. After administrating 12 weeks, observed the value of bcl -2, bax and caspase -3 in the tissue of corpus cavernosum smooth muscle of DMED - rats. Results ： The reinforcing kidney and activating blood recipe can improve the vulue of bcl - 2, bax and caspase -3. Conclusion： The reinforcing kidney and activating blood recipe has regulatory function for bcl -2, bax and caspase - 3 in the tissue of corpus cavernosum smooth muscle of DMED - rats.