褐藻77K荧光特异性研究

STUDIES ON CHARACTERIZATION OF FLUORESCENCE EMISSION SPECTRA OF BROWN ALGAE AT 77 K

测定了裙带菜、叉开网地藻、海带、囊藻、海蒿子、鼠尾藻、萱藻和水云等８种褐藻的７７Ｋ荧光光谱并同菠菜和红藻条斑紫菜作了比较。结果表明与红藻和高等植物明显不同，褐藻没有作为ＰＳＩ特征的７３０ｎｍ荧光峰。按荧光主峰的波长，可以分为二种类型：裙带菜、叉开网地藻、海带和囊藻的荧光主峰位于６９０ｎｍ，海蒿子、萱藻、水云和鼠尾藻的荧光主峰在７０５～７２０ｎｍ。这种７７Ｋ荧光特异性预示褐藻同高等植物之间在ＰＳＩ结构上的差异。

Fluorescence emission spectra of brown algae Undaria pinnatifida, Dictyopteris divaricata, Laminaria japonica, Colpomenia sinuosa, Sargassum confusum, Sargassum thunbergii, Scytosiphon lomentarius and Ectocarpus confervoides were determined and compared to the higher plant Spinacia oleracea and red alga Porphyra yezoensis at 77 K. The results showed that brown algae were significantly different from higher plant and red alga, they had no fluorescence emission at 730 nm which is taken as the characteristic of PSI. The spectra were divided into two types according to the wavelength of major fluorescence emission. It was at 690 nm in Undaria pinnatifida,Dictyopteris divaricata,Laminaria japonica,Colpomenia sinuosa and at 705～720 nm in Sargassum confusum,Sargassum thunbergii,Scytosiphon lomentarius, Ectocarpus confervoides. The characterization of fluorescence at 77 K may foresee the difference of PSI structure between brown algae and higher plant.