马桑内酯所致慢性癫痫大鼠海马星形胶质细胞胶质纤维酸性蛋白的表达

GLIAL FIBRILLARY ACIDIC PROTEIN EXPRESSION OF HIPPOCAMPAL ASTROCYTES IN CHRONIC MODEL RAT WITH EPILEPSY INDUCED BY CORIARIA LACTONE

用免疫组织化学方法研究了系统应用马桑内酯所致的慢性癫痫大鼠海马中星形胶质细胞的胶质纤维酸性蛋白的表达。结果证明，整个海马的胶质纤维酸性蛋白免疫反应明显增强，并可见阳性细胞增生、胞体肥大，尤以齿状回门区和海马分子腔隙层及始层为甚。此外，本实验还发现海马胶质纤维酸性蛋白的阳性反应强度随发作后不同时间间隔（２ ｈ～９ ｄ）而不同，且直至发作后９ ｄ，胶质纤维酸性蛋白阳性反应程度仍高于对照组。此结果表明，马桑内酯所致癫痫反复发作可使海马星形胶质细胞内胶质纤维酸性蛋白的表达长期保持在较高水平。

Glial fibrillary acidic protein(GFAP) expression of hippocampal astrocytes in chronic model rats with epilepsy kindled by systemic use of Coriaria Lactone was studied with immunohistochemistry method. Obvious increase in staining intensity, proliferation and hypertrophy of GFAP positive astrocytes were found in whole hippocampus, especially in the hilar area, stratum moleculo lacunosum and stratum oriens of the rats with epilepsy. In addition, the staining intensity of GFAP staining changed with different time interval(2 hours～9 days) after seizures, but by 9 days after seizure, the staining intensity remained higher than the control rat. These results indicate that repeated seizure episodes induced by Coriaria Lactone may keep the GFAP expression in hippocampal astrocytes at a high level for a long time. (Figures 1～8 on plate 25)