Rho激酶抑制剂Y-27632对涎腺腺样囊性癌细胞生长增殖影响的研究

Effects of Rho-kinase inhibitor Y-27632 on the development and proliferation of adenoid cystic carcinoma cells

目的观察Rho激酶抑制剂Y-27632对涎腺腺样囊性癌细胞肺高转移细胞株(ACC-M)生长增殖的影响,探讨Y-27632对涎腺腺样囊性癌可能疗效,以期为治疗涎腺腺样囊性癌提供新思路。方法 2007年10月至2008年2月在浙江大学医学实验室,形态学观察Rho激酶抑制剂Y-27632作用下ACC-M细胞的生长变化,并用四甲基偶氮唑蓝(MTT)比色法检测不同药物浓度下ACC-M的增殖情况。结果抑制剂Y-27632作用下ACC-M细胞变成梭形,细胞长轴增加,细胞间连接消失,细胞膜不规则,皱缩不整齐,边界有丝状突起,有倾向凋亡的趋势;MTT比色法检测结果发现,不同浓度Y-27632作用下检测到的ACC-M细胞光密度值均比对照组小,且当药物浓度为10μmol/L时抑制作用有统计学意义(P<0.01)。结论 Rho激酶抑制剂Y-27632在体外实验中对涎腺腺样囊性癌细胞的生长增殖起一定的抑制作用。

Objective To investigate the effects of Rho-kinase inhibitor Y-27632 on the development and proliferation of adenoid cystic carcinoma cells,so as to provide new ideas for the treatment of adenoid cystic carcinoma.Methods Morphology change：observe the ACC-M cells after 10 μmol/L Y-27632 added in medium;Methabenzthiazuron tetrazolium（MTT）：ACC-M exposed to various concentration of Y-27632（0、2.5、5、10、20 μmol/L）24 hours,then terminate and detect the OD value.Results The morphology changed a lot in cells of ACC-M when exposed to 10 μmol/L Y-27632.With MTT we found cell proliferation of ACC-M was inhibited significantly at 10 μmol/L（P 0.01）.Conclusion The inhibitor Y-27632 can do some inhibition to the proliferation of ACC in vitro.