加味四逆散对血吸虫病小鼠肝纤维化相关因子表达的影响

Effect of Modified Sini Powder on Expression of Liver Fibrosis Related Factors in Schistosomiasis Mice

目的观察加味四逆散对血吸虫病肝纤维化小鼠透明质酸(HA)、血清Ⅲ型前胶原(PCⅢ)、羟脯氨酸(Hyp)及转化生长因子-β1(TGF-β1)mRNA表达的影响。方法 NIH小鼠经皮肤人工感染血吸虫尾蚴后随机分为4组:模型组(B组)、吡喹酮组(C组)、吡喹酮+加味四逆散组(D组)、吡喹酮+秋水仙碱组(E组),同时设正常对照组(A组)。病理观察小鼠肝组织,于肝纤维化形成后第21日开始灌服相应药物及生理盐水,C、D、E组在第45日时予以吡喹酮一次性灌胃进行杀虫,第78日小鼠心脏采血用放免法测定血清PCⅢ和HA含量,取肝组织匀浆测定Hyp含量;RT-PCR检测TGF-β1 mRNA的表达。结果 HA水平D组高于A组,低于B、C、E组(P<0.01,P<0.05);PCⅢ水平D组低于B、C、E组(P<0.05);Hyp水平D组高于A组,低于B、C、E组(P<0.05);TGF-β1 mRNA表达D组低于B、C、E组(P<0.05)。结论加味四逆散联合吡喹酮能有效改善血吸虫病小鼠肝纤维化,降低TGF-β1 mRNA的表达。

Objectives To evaluate the effect of Modified Sini Powder on the hepatic expression of hyaluronic acid （HA）, procollagen peptide （PC III）, hydroxyproline （Hyp） and transforming growth factor-β （TGF-β1） in a routine model of liver fibrosis induced by Schistosoma japonicum infection. Methods The NIH mice were successfully induced to liver fibrosis by infection with Schistosoma japonicum and then randomly divided into model group （group B）, praziquantel group （group C）, praziguanted plus Modified Sini Powder group （group D） and praziquantel plus colchicine group （group E）. The mice in groups C, D and E were treated with praziquantel, praziquantel plus Modified Sini Powder and praziquantel plus colchicine, respectively. In addition, a group of unfected NIH mice （group A） served as normal control. Histological specimens were stained with HE. Expression of TGF-β1 mRNA was determined by RT-PCR and the content of Hyp in liver was measured by ELISA. Radioimmtanoassay technique was employed to detect the serum content changes of HA and PCIII. Results The levels of HA, PCIII, Hyp and TGF-β1 mRNA in group D were markedly lower than those in group B, C and E （P〈0.05）. Conclusion Modified Sini Powder combined with praziquantel can implove liver fibrosis due to Schistosoma japonicum, perhaps by down-regulating the expression of TGF-β1 mRNA.