高效液相色谱法测定排石利胆片中芍药苷的含量

Content Determination of Paeoniflorin in Paishilidan Tablets by HPLC

目的建立排石利胆片中芍药苷含量的测定方法。方法采用高效液相色谱法,色谱柱:Hypersil ODS-3 C18(4.6 mm×150 mm,5μm);流动相:乙腈-0.1%磷酸溶液(18∶82);检测波长为230 nm,流速1.0 mL/min。结果芍药苷在0.06～0.36μg范围内与峰面积呈良好线性关系,r=0.999 9,平均回收率为99.77%,RSD=1.35%(n=6)。结论该方法简单、快速、准确,可用于该制剂中芍药苷的质量控制。

Objective To establish a method for determination of paeoniflorin in Paishilidan tablets by HPLC. Method Hypersil ODS-3 C18 column （4.6 mm × 150 mm, 5 um） was used, acetonitrile-0.1% phosphoric acid （18 ： 82） was the mobile phase, detection wavelength was at 230 nm, and the flow rate was 1.0 mL/min. Result The linear range of paeoniflorin was 0.06-0.36 ug, r=0.999 9, and the average recovery was 99.77% with RSD of 1.35% （n =6）. Conclusion The method was simple, quick and accurate, and available for quality control of the preparation.