430例婴幼儿眼分泌物细菌的分离与药敏分析

Study of pathogens isolated from 430 infants eye secretion and the ir drug susceptibility test

目的了解本院婴幼儿感染性眼部疾病致病细菌分布情况及其耐药现状，为临床治疗婴幼儿眼部感染提供实验依据。方法统计广东省妇幼保健院2008年7月～2010年5月眼科门诊和病房430例疑似眼部感染疾病婴幼儿眼分泌物标本的细菌分离培养和药敏试验结果。鉴定和药敏试验采用Biomerieux VITEK2 Compact拿自动细菌分析仪进行分析。结果430份标本中分离出致痫菌97株，阳性率为22．6％，革兰阳性球菌54株占55．7％；革兰阴性菌43株占443％，主要以金黄色葡萄球菌为主，大肠埃希菌次之。其中，葡萄球菌对万古霉素、莫西沙星、环丙沙星、奎诺普汀，达福普汀、利奈唑烷的耐药率为0；青霉素耐药率最高，为97．78％。革兰阴性杆菌敏感率较高的是喹诺酮类抗生素。结论万古霉素、莫西沙星、环丙沙星、利奈唑烷、喹诺酮类抗生素是跟部细菌感染治疗的首选药物，临床应根据不同的患者选用合适的抗生素进行治疗。

Objective This study is to investigate bacteria spectrum in the secretion of infants＇eves and their drug tolerance against antibioties in order to provide basis for clinicians to select antibiotics rationally. Methods Results of the bacterimn culture and drug susceptibility test from the eye seeretion of 430 infantswith ophthalmia from ophthatmologic clinic in Guangdong Wonmen and children＇s hospital and heatlh institute were summarized from july, 2008 to may, 2010. Biomerieux V ITEK2 Compact Automatic analyzer bacteria were used to identify the bacterias and do the drug suseeptibility test. Results Bacterium growth was found in 97case and the positix e rate was 22.6%.Cram positive bacteria were detected in 54 case （ 55.7% ）, Gram negative bacteria were detected in 43 cases ;（ 44.3% ）.About the main is Staphylococcus aureus the second bacteria is Eseherichieoli.Staphylococcus had the lowest drug tolerance tovanccmyein , Moxifloxacin, CIP,.Linezolid; Staphylococcus was resistive to penieillin, drugresistanee rate is 97.78%.Cram negative bacteria had lower drag tolerance to Quinolones.Conclusion vanccmyein , Moxifloxaein, CIP,.Linezolid, Quinolones is the first choice to treat the ocu-lar bacterial infections. Clinicans should selected suitable antibiotie to treat the different patient.