固相萃取-高效液相色谱法测定人血浆中雷尼替丁浓度

Dertermination of Ranitidine in Human Plasma by Solid phase Extraction and HPLC

目的 建立测定人血浆中雷尼替丁浓度的HPLC-UV方法.方法 血浆经C18柱固相萃取,洗脱液在高效液相色谱仪上测定.结果 血浆雷尼替丁浓度在10.0～1000.0ng·ml-1之间线性关系良好,相关系数r2为0.999.日内变异和日间变异在3.6%之内,萃取回收率在87.2%～96.2%之间.结论 本方法操作简便、快速、准确,适合于雷尼替丁的人体药代动力学研究.

Objective To make a sensitive method for the determination of ranitidine in human plasma by HPLC-UC. Methods Plasma samples were extracted with C18 Solid phase columns. Chromatograph analysis was carried out with a C18 column and UV Detector which was set at 316 nm. Results Interior impurity in human plasma does not interfere with the determination of ranitidine and internal standard. Good linear relationship（r2=0.999） was obtained in the concentration range from 10.0 to 1000.02%. Conclusion This method is sensitive, selective and simple, is suitable for clinical pharmacokinetic and bioequivalent study of ranitidine.