五味子乙素对染矽尘大鼠血清唾液酸和铜蓝蛋白的影响

Effects of schisandrin B on serum sialic acid and ceruloplasmin in rat lungs exposed to silica

目的观察五味子乙素(schisandrin B,Sch-B)对染矽尘大鼠血清唾液酸(sialic acid,SA)和铜蓝蛋白(cerulopasmin,CP)的影响。方法将48只大鼠随机分为对照组、染矽尘组、Sch-B干预组,每组16只。采用暴露式气管内注入法给染矽尘组和Sch-B干预组大鼠一次性染矽尘(50 mg/只),同法对照组大鼠给予生理盐水(1 ml/只)。染尘后第1天开始灌胃给药,Sch-B干预组给予Sch-B 80 mg/(kg.d),其他组给予等体积的橄榄油。药物干预7 d和28 d后,各组分别取8只大鼠处死,取其肺组织光镜观察其病理切片;紫外分光光度计检测血清CP和SA的含量。结果对照组肺组织结构基本正常,染矽尘组肺损伤明显,Sch-B组较轻。给药后7 d,各组之间大鼠血清SA和CP含量均无统计学差异(P>0.05);28 d时,染矽尘组血清中SA和CP含量显著高于对照组,差异有统计学意义(P<0.01),Sch-B组显著低于染矽尘组,差异有统计学意义(P<0.05)。结论 Sch-B能抑制染矽尘大鼠血清中SA含量和CP活性的升高。

Objective To investigate the effects of schisandrin B（Sch-B）on serum sialic acid and cerulopasmin in rats with silicosis.Methods Forty-eight rats were randomly divided into control group（n=16）,silica group（n=16）and Sch-B group（n=16）.The 50 mg silicon dioxide was injected intratracheally in every rat in silica group and Sch-B group,while 1 ml physiologic saline was injected intratracheally in every rat in control group.From the first day after injection,rats were orally given Sch-B 80 mg/（kg · d） in Sch-B group,while the same dose of olive oil was given in control group and silica group.After treatment for 7 d and 28 d,eight rats in each group were sacrificed and samples were collected.The serum sialic acid content and cerulopasmin activity were assayed by a spectrophotometer.The lung histopathological changes were observed after HE staining.Results HE staining showed that the lungs were normal in control group,while the lungs were damaged obviously in silica group and slightly in Sch-B group.At 7 d,the content of sialic acid and the activity of cerulopasmin had no significant difference among 3 groups（P〉0.05）.At 28 d,the content of SA and the activity of CP were significantly higher in silica group than in control group（P〈0.01）,and they were significantly lower in Sch-B group than in silica group（P〉0.05）.Conclusion Sch-B could inhibit the increase of serum SA content and CP activity.