摘要
利用Plackett-Burman设计和响应面设计方法对Hd3菌株的发酵条件进行了优化研究,确定了最佳培养基组成及培养条件.试验结果表明：Hd3菌株的摇瓶培养基配方为葡萄糖24.71 g/L,MgSO4.7H2O 1.08 g/L,土豆244.17 g/L.发酵条件为pH 6~7,250 mL三角瓶装90 mL发酵液,接入9×103cfu/mL菌量,31℃培养9 d.
The components of liquid medium for strain Hd3 were studied by Plackett-Burman design and response surface methodology and their fermentation conditions including culture temperature,culture period,the pH value of medium,inoculate concentration and the volume of liquid medium of strains were optimized.The studied results of strain Hd3 showed that the suitable liquid medium was composed of 24.71 g/L glucose,1.08 g/L MgSO 4·7H 2O,244.17 g/L potato and distilled water;and the suitable cultivated condition was 31 ℃,9 d,9×103 cfu/mL and 90mL liquid medium in 250 mL flask,pH 6~7.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第1期148-153,共6页
Journal of Southwest China Normal University(Natural Science Edition)
基金
公益性行业(农业)科研专项经费资助项目(nyhyzx07-048)
关键词
内生真菌
发酵
优化
响应面
endophytic fungus
fermentation
optimization
response surface
