甘蓝型油菜雄性不育的酶活性和同工酶谱分析

ANALYSIS OF ENZYMATIC ACTIVITY AND ISOENZYMES OF MALE STERILITY IN RAPESEED (BRASSICA NAPUS L.)

对甘蓝型油菜细胞质雄性不育（ＣＭＳ）系Ｌ１７Ａ及其保持系Ｌ１７Ｂ，细胞核雄性不育（ＧＭＳ）的不育株Ｌ１８Ａ和可育株Ｌ１８Ｂ以及细胞核＋细胞质双重不育（ＧＣＤＭＳ）的核不育株ＧＣＤＭＳ－ＨＡ和质不育株ＧＣＤＭＳ－ＢＡ的酶活性和同工酶谱差异研究表明，３种类型不育系都随着花蕾的发育，ＰＯＤ酶活性均无明显变化，ＣＡＴ酶活性则显著降低。ＧＣＤＭＳ－ＨＡ的ＰＯＤ酶活性与Ｌ１８Ａ相近，极显著高于Ｌ１７Ａ和Ｌ１７Ｂ，显著高于ＧＣＤＭＳ－ＢＡ和Ｌ１８Ｂ；Ｌ１７Ａ的ＣＡＴ酶活性与ＧＣＤＭＳ－ＨＡ相近，极显著高于Ｌ１７Ｂ和Ｌ１８Ａ，显著高于ＧＣＤＭＳ－ＢＡ和Ｌ１８Ｂ。在花蕾发育早期，ＣＭＳ系和ＧＣＤＭＳ系之间ＰＯＤ酶同工酶谱无明显差异，ＧＣＤＭＳ－ＨＡ和ＧＣＤＭＳ－ＢＡ的ＰＯＤ酶活性较ＣＭＳ系和ＧＭＳ系弱。花蕾发育后期，各材料之间ＰＯＤ酶同工酶谱和酶活性都相近。在小花蕾中，ＥＳＴ酶同工酶谱ＣＭＳ与ＧＭＳ和ＧＣＤＭＳ显著不同，前者较后者多３条慢带；ＧＭＳ有１条泳动速度中等的带比ＣＭＳ和ＧＣＤＭＳ具有较强的活性。随着花蕾的发育，６个材料之间ＥＳＴ酶同工酶谱和活性趋于一致，仅迁移率存在差异。ＣＭＳ，ＧＭＳ和ＧＣＤＭＳ的酶活性和同工酶谱?

Differences were analysed among the CMS line L17A and its maintainer line L17B, the sterile plant L18A and the fertile plant L18B of the GMS line L18AB and the genic sterile plant (GCDMS HA) and cytoplasmic sterile plant (GCDMS BA) of GCDA 1, a genic and cytoplasmic double MS (GCDMS) line in their enzymatic activity and isoenzyme patterns. CAT activity decreased significantly with bud development in all the three types of male sterility, while POD activity remained little changed. POD activity in GCDMS HA was similar to that in L18A, being highly significantly stronger than that in L17A and L17B and significantly higher than that in GCDMS BA and L18B. CAT activity in L17A was same as that in GCDMS HA, being highly significantly stronger than that in L17B and L18A and significantly higher than that in of GCDMS BA and L18B. In the early period of bud development, no significant difference was observed between CMS and GCDMS in their isoenzyme patterns though POD activity appeared low in GCDMS HA and GCDMS BA as compared with that in CMS and GMS lines. In the late period of bud development, the isoenzyme patterns and activity of POD became quite similar in all genotypes. The isoenzyme patterns of EST of the CMS lines were markedly distinguishable from those of the GMS or GCDMS lines, the former having three slowly moving bands in the small buds while the latter having none. A band with medium moving speed was noticed in the EST enzyme pattern of GMS, which had a higher activity than that in CMS or GCDMS lines. However, more similarity appeared as the bud grew, and their differences were manifested only in migration rate. The expression of POD and EST enzymatic activity and isoenzyme patterns of CMS, GMS and GCDMS indicate that interactions exist between the GMS and CMS sterile gene systems.