水稻谷胱甘肽过氧化物酶的测定法

DETERMINATION OF GLUTATHIONE PEROXIDASE IN RICE SEEDLINGS

以水稻幼苗的叶片为材料，用０．２ｍｏｌ／ＬｐＨ６．２的磷酸缓冲液（含１ｍｍｏｌ／ＬＥＤＴＡ－２Ｎａ，５％的水溶性ＰＶＰ）作为谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）的提取介质，偏磷酸为酶促反应的蛋白质沉淀剂，二硫代对二硝基苯甲酸（ＤＴＮＢ）与ＧＳＨ显色反应３ｍｉｎ，在４１２ｎｍ测定酶管和非酶管的ＯＤ值，以测定谷胱甘肽过氧化物酶的活性。

In the present experiment, 0.2 M PBS (pH 6.2) containing 1 mM EDTA 2Na and 5% PVP was shown to be suitable for the extraction of GSH Px in the leaves of rice seedlings, and HPO 3 was a good protein precipitant in enzyme reactions. The activity of GSH Px could be calculated by determining the optical density (OD) of the enzyme tube and the nonenzyme tube at 412 nm of wave length after GSH had reacted with DTNB for 5 minutes.