摘要
黑生１０１ 是利用花粉管通道技术育成的第一个大豆品种，利用１３２ 个随机引物对黑生 １０１ 及其供体、受体的 Ｄ Ｎ Ａ 进行 Ｒ Ａ Ｐ Ｄ扩增，其中３ 个单引物和２个双引物组合起来，均能在后代黑生 １０１ 中扩增出与供体相同而与受体不同的 Ｄ Ｎ Ａ 片段，证明黑生１０１ 中确有供体 Ｄ Ｎ Ａ 片段的导入。
Heisheng 101 was the first soybean cultivar obtained from pollen tube channel. We used RAPD to amplify the total DNA of Heisheng 101 and its donor and recipient by 132 random primers. The DNA polymorphism results of RAPD molecular verification indicated :Either 3 single random primer or 2 double primers combinations can be amplified polymorphic DNA fragments from Heisheng 101. The results proved that Heisheng 101 was truly obtained from the transformation progeny of its donor DNA fragments introduced into recipient.
出处
《大豆科学》
CAS
CSCD
北大核心
1999年第3期230-235,共6页
Soybean Science
基金
自然科学基金
