摘要
运用Ｍａｎｎｉｃｈ反应，将褪黑激素与ＢＳＡ联结起来制备完全抗原，用ＵＶ－２６０紫外仪测定ＭＬＴ与ＢＳＡ的联结比为１２∶１。用此抗原免疫新西兰大耳兔５只，经过６次免疫后，发现抗体效价平均１∶１００００（ＥＬＩＳＡ）。将抗血清经Ｎａ２ＳＯ４沉淀、Ｓｅｐｈａｄｅｘ－Ｇ－２００过柱层析、收集纯化抗体，用分光光度计７２１测其蛋白浓度。经５－羟色胺等阻断试验发现抗体特异性较高。
Melatonin complete antigen is successfully synthesized through mannich formaldehyde condensation reaction with molar ratio of melatonin to BSA 12∶1. Five New Zealand Rabbits were immunized against the antigen,with the average of antiserum titers of 1∶10000. The antiserum were treated with Na 2SO 4 and Sephadex-G-200. The purified antibody is collected and with UV-721 table Spectrophotometrically,with no cross reactions for 5 methoxy tryptophan.
