高效毛细管电泳法同时检测鸡肝中氟喹诺酮类和磺胺类药物残留

Simultaneous Determination of Residual Fluoroquinolones and Sulfonamides in Chicken Liver by High Performance Capillary Electrophoresis

以空白鸡肝为试材,以高效毛细管电泳法为检测手段,建立了高效毛细管电泳（HPCE）法检测鸡肝中3种喹诺酮类、2种磺胺类等合成抗菌剂残留的方法。结果表明：样品经二氯甲烷提取,正己烷脱脂,旋转蒸发浓缩后,在pH 8.48的40 mmol·L^-1磷酸氢二钠-20 mmol.L-1柠檬酸缓冲体系中,紫外检测波长262 nm,检测电压22 kV,检测温度25℃的电泳条件下,3种氟喹诺酮类和2种磺胺类药物在10 min内能够完全分离。5种抗生素在鸡肝组织中的回收率为85.39%-90.23%,RSD为2.95%-3.41%,且各组分浓度与峰面积呈良好的线性关系。

A procedure is developed for the simultaneous determination of fluoroquinolones（FQs）and sulfonamides（SAs）.The antibiotics in the samples were extracted by dichloromethane,and defatted with hexane,concentrated by rotary evaporation,then determined by HPCE at 22 kV of separation voltage and 262 nm detection wave length,and column temperature 25℃,in 40 mmol·L-1Na2HPO4-20 mmol·L-1citric acid running buffer（pH 8.47）,the five remedies could be well separated within 10 min.Each had a good linear relationship.The average recoveries from laboratory spiking experiments were 85.39%-90.23%,the relative standard deviations（RSD）were in the range of 2.95%-3.41%.