COPD患者呼出气冷凝液中8-异前列腺素的检测及其意义

Clinic significances of 8-isoprostane in Exhaled Breath Condensate in COPD

目的研究COPD患者呼出气冷凝液(EBC)中8-异前列腺素(8-isoPG)浓度的改变及临床意义。方法收集COPD患者(39例)急性发作期和缓解期以及正常对照组(32例)的EBC,用酶标记法检测EBC中8-isoPG,同时检测AECOPD患者的第一秒呼气容量(FEV1)、最大呼气流速(PEF)、pH值、PaCO2、PaO2及血白细胞总数。结果①COPD患者急性发作期8-isoPG为6.44±3.68 ng/L,高于缓解期(4.04±1.25 ng/L)及正常对照组(3.31±0.91 ng/L),P<0.05;②COPD患者8-isoPG浓度与痰量呈正相关,r=0.217,P<0.05。结论 COPD的急性发作期,8-isoPG升高反映了氧化应激增强。

Objective To study the changes and clinic significances of 8-isoprostane（8-isoPG）in exhaled breath condensate（EBC）in patients of chronic obstructive pulmonary disease（COPD）.Methods EBC of 39 cases in acute episode COPD（AECOPD）and period of remission,and that of 32 normal person were collected.EIA enzyme labeling regulation was used for assaying the density of 8-isoPG in EBC.Forced expiratory volume in one second（FEV1）and peak expiratory flow（PEF） of AECOPD patients were observed at the same time,and pH,PaCO2,PaO2,SaO2,leukocyte were assayed also.Results ① The concentration of 8-isoPG in EBC in AECOPD was 6.44±3.68 ng/L,and that was 4.04±1.25 ng/L in remission of COPD and that was 3.31±0.91 ng/L in normal controls,P0.05.② The concentration of 8-isoPG in EBC had positive relationship with the volume of sputum in AECOPD patients,r=0.217,P0.05.Conclusions The concentration of 8-isoPG in EBC in AECOPD patients were raised when oxidation stress was reinforced.