大火草根的化学成分研究

Study on Chemical Constituents of the Root of Anemone tomentosa

目的研究大火草根的化学成分。方法采用硅胶、Sephadex LH-20柱色谱和薄层色谱等方法对甘肃产的大火草根的化学成分进行分离提纯,运用波谱分析技术和理化性质对照等方法进行结构鉴定。结果分离得到8个非生物碱类化合物,分别鉴定为4,5-二甲氧基-7-甲基香豆素(1)、4,7-二甲氧基-5-甲基香豆素(2)、白桦脂酸(3)、齐墩果酸(4)、齐墩果酸3-O-α-L-吡喃阿拉伯糖苷(5)、齐墩果酸3-O-β-D-吡喃半乳糖基-(1→3)-β-D-吡喃葡萄糖苷(6)、常青藤皂苷元3-O-α-L-吡喃阿拉伯糖苷(7)和18-羟基乌索酸(8)。结论除化合物1、2和4外,其余5个化合物均为首次从该植物中分离得到,其中化合物6～8为首次从五加属植物中分离得到。

OBJECTIVE To study the chemical constituents of the root of Anemone tomentosa. METHODS The chemical constituents were isolated and purified by silica gel, Sephadex LH -20 column and thin-layer chromatography. Their structures were elucidated by spectral analysis and physico-chemical properties. RESULTS Eight non-alkaloid compounds were isolated and identified as 4,5 -dimethoxyl-7-methylcoumarin （ 1 ）, 4,7-dimethoxyl-5-methyl coumarin （ 2 ）, betulinic acid （ 3 ）, oleanolic acid （ 4 ）, oleanolic acid 3 - O-α-L-arabinopyranoside （ 5 ）, oleanolic acid 3-O-β-D-galactopyranosyl- （ 1 → 3 ） -fi-D-glucopyranoside （ 6 ）, hederagenin 3-O-α-L-arabi- nopyranoside （7）and 18-hydroxyursolic acid （8）. CONCLUSION Five compounds except 1,2 and 4 were obtained from A. tomentosa for the first time. Compounds 6 - 8 were isolated from Anemone genus for the first time.