UPLC-MS/MS测定康艾注射液中5种主要成分含量

UPLC-MS/MS Determination of Five Main Components in Kangai Injection

目的建立同时测定康艾注射液中黄芪甲苷、人参皂苷Rg1、Re、Rf、Rb1含量的超高效液相-串联四级杆质谱方法。方法多反应监测模式测定,以Waters ACQUITY UPLC(BEHC18进行分离,乙腈-20 mmol.L-1醋酸铵溶液梯度洗脱,流速:0.4mL.min-1。离子化模式:ESI+。结果黄芪甲苷线性范围在5.0～500.0μg.L-1之间,人参皂苷Re和Rg1线性范围在0.2～20.0 mg.L-1之间,人参皂苷Rf和Rb1线性范围在10.0～1 000.0μg.L-1之间,r分别在0.991 5～0.995 2之间,检出浓度分别在1.57～67.4μg.L-1之间,回收率分别在95.6%～107.0%之间。结论该方法快速、准确、灵敏,可作为康艾注射中5种主要成分同时测定的方法。

OBJECTIVE To establish a method for the determination of 5 main components in Kangai Injection by UPLC triplequadrupole tandem mass spectrometer. METHODS Multi-reactions monitoring（MRM） technology was used. The separation was performed on a Waters ACQUITY UPLC BEH Cls column with acetonitrile -20 mmol · L^-1 ammonium acetate as the mobile phase at a flow rate of 0.4 mL · min^- 1. ESI was used for ionigation. RESULTS The standard curve of the astragaloside showed good linearity over the concentration range of 5.0 - 500. 0 μg · L^- 1, The standard curves of the ginsenoside R, and Rg1 showed good linearity over the concentration range of 0. 2 - 20. 0 mg · L^-1. The standard curves of the ginsenoside Rf and Rbl showed good linearity over the concen- tration range of 10. 0 - 1 000. 0 μg · L^-1. The correlation coefficients were beteewn 0. 991 5 and 0. 995 2. The detection limits of the 5 main components were between 1.57 and 67.4 μg · L^-1 , and the average recoveries were 95.6% - 107. 0%. CONCLUSION The method has the advantages of rapidness, high precision and high sensitivity. It is suitable for the determination of 5 main components in Kangai Injection.