摘要
用低聚半乳糖对牛乳β-乳球蛋白进行糖基化处理,通过间接ELISA以及动物试验检测该结合产物的抗原性和免疫原性,结果表明糖基化处理能有效降低牛乳β-乳球蛋白的抗原性和免疫原性。通过动物试验和双抗体夹心ELISA方法检测牛乳β-乳球蛋白诱导产生IgE的量,结果表明低聚半乳糖糖基化对牛乳β-乳球蛋白诱导产生IgE具有显著抑制作用。
Bovine milk β-Lactoglobulin was conjugated with galactooligosaccharide.Antigenicity and immuno-genicity of the conjugates were measured by indirect ELISA assay and animal experiments.The results showed that conjugation with galactooligosaccharide can reduce the antigenicity and immunogenicity of bovine milk β-Lactoglobulin effectively.Besides,animal experiments and sandwich ELISA method were used to measure the IgE level in vivo,induced by β-LG and its glycosylation product.The result indicated that conjunction with galactooligosaccharide suppressed IgE production by bovine milk β-Lactoglobulin significantly.
出处
《食品科技》
CAS
北大核心
2011年第2期5-8,共4页
Food Science and Technology
基金
"十一五"国家科技支撑计划子课题(2006BAD04A06)
教育部<留学回国人员科研启动基金>项目
北京林业大学国家大学生创新性实验计划院级项目
